Titration Fundamentals

Determine the optimal antibody concentration for your flow cytometry panel. Enter your antibodies below to generate a tube layout, serial dilution steps, and a downloadable protocol PDF.

Serial dilution calculatorTube layout generatorPDF downloadStain Index guidance

Setup

Titration points:

Viability reagent:

Exported by:

Antibody 1

Antibody *

Clone

Fluorophore

Catalog #

ug / mL

Lot #

Highest test vol (uL) *

Incubation vol (uL) *

Cells / tube

Fields marked * are required to generate the tube layout and steps. Use the same incubation volume and cells/tube for all antibodies in a single experiment.

Scientific Background

References

•Burn GL, et al. (2024) Combinatorial antibody titrations for high-parameter flow cytometry. Cytometry Part A. Wiley. [link]
•Becton Dickinson. How to Titrate Antibodies for Flow Cytometry. bdbiosciences.com [link]
•BioLegend. Antibody Titration Protocol. biolegend.com [link]
•Ferrer-Font L, et al. (2020) The Power of Reagent Titration in Flow Cytometry. Cells 9(5), 1022. MDPI. [link]
•Perfetto SP, Chattopadhyay PK, Roederer M (2004) Seventeen-colour multiparameter flow cytometry. Nature Reviews Immunology 4, 648–655. [link]