TiterGuide
Practical education for flow cytometry: from titration basics to advanced panel design, grounded in real experimental data.
Titration fundamentals
How to determine the optimal antibody concentration: serial dilution planner, tube layout generator, stain index guidance, and PDF download.
Panel design principles
Fluorochrome selection, spectral overlap, and brightness matching for multicolor experiments.
Stain index & signal-to-noise
Understanding and interpreting SI and S/N ratios to compare antibody performance across lots.
Sample preparation
Cell viability, fixation protocols, and best practices for PBMC and tissue samples.
Fluorophore naming conventions
What the number in BV421, Alexa Fluor 647, and SuperBright 600 actually means: excitation vs. emission convention decoded by brand family.
Tandem dye naming cheat sheet
Every APC, PE, and PerCP tandem variant mapped by emission peak with cross-vendor equivalents and similar-sounding dye warnings.
Vendor fluorophore resources
Curated links to fluorophore guides, spectrum viewers, and lot-specific COA lookup tools for BD, BioLegend, Cytek, Miltenyi, Invitrogen, and more.
Ready to start? The data is already here.
Browse thousands of real titration results in TiterFinder, then dive into any guide above.