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CD8 · RV828

Dataset ID: GSYU1091 • DOI: 10.64157/GSYU1091

Experiment Information

NameJames Marvin
Emailjmarvin@cores.utah.edu
Employer / AffiliationUniversity of Utah
Month of analysisMarch
Year of analysis2026
Collection SoftwareSpectroFlo
Analysis SoftwareFlowJo
Plug-ins, other analysis toolsnone
ReferencesCombinatorial antibody titrations; Cytometry. 2024; 105(5): 388–393.
Quality control measuresSpectroFlo QC beads lot 2006
AntigenCD8
CloneRPA-T8
ConjugateRV828
Target SpeciesHuman
Antibody host speciesMouse
IsotypeIgG1,k
Clonalitymonoclonal
SupplierBD
Catalog #572638
Lot #5300444
Concentration for this Lot #100 µg/mL
Other antibodies in tubenone
Instrument ManufacturerCytek
InstrumentationAurora
Lasers (nm)355 nm, 405 nm, 488 nm, 561 nm, 640 nm
Incubation Time45 minutes
Temperature (°C)20
Incubation volume (µL)100 µL
GatingFSC-A × SSC-A
Tissueblood
Cell typelymphocytes
Preparation protocolRBC lysis
Cell count2,000,000
Fresh or FrozenFresh, never frozen, incubated with antibody day of collection
Live or FixedLive at the time of antibody incubation
Fixed after antibody incubation?No, no fix after antibody incubation

Figure

Titration figure for GSYU1091

Files

0.01.fcs, 0.02.fcs, 0.04.fcs, 0.078.fcs, 0.156.fcs, 0.3125.fcs, 0.625.fcs, 1.25.fcs, 2.5.fcs

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DOI

https://doi.org/10.64157/GSYU1091

Citation

James Marvin. (2026). CD8 RV828 Titration Dataset. TiterPubs. https://doi.org/10.64157/GSYU1091