CD8 · RV828
Dataset ID: GSYU1091 • DOI: 10.64157/GSYU1091
Experiment Information
- Name
- James Marvin
- Employer / Affiliation
- University of Utah
- Month of analysis
- March
- Year of analysis
- 2026
- Collection Software
- SpectroFlo
- Analysis Software
- FlowJo
- Plug-ins, other analysis tools
- none
- References
- Combinatorial antibody titrations; Cytometry. 2024; 105(5): 388–393.
- Quality control measures
- SpectroFlo QC beads lot 2006
- Antigen
- CD8
- Clone
- RPA-T8
- Conjugate
- RV828
- Target Species
- Human
- Antibody host species
- Mouse
- Isotype
- IgG1,k
- Clonality
- monoclonal
- Supplier
- BD
- Catalog #
- 572638
- Lot #
- 5300444
- Concentration for this Lot #
- 100 µg/mL
- Other antibodies in tube
- none
- Instrument Manufacturer
- Cytek
- Instrumentation
- Aurora
- Lasers (nm)
- 355 nm, 405 nm, 488 nm, 561 nm, 640 nm
- Incubation Time
- 45 minutes
- Incubation temperature (°C)
- 20
- Incubation volume (µL)
- 100 µL
- Gating
- FSC-A × SSC-A
- Tissue
- blood
- Cell type
- lymphocytes
- Preparation protocol
- RBC lysis
- Cell count
- 2,000,000
- Fresh or Frozen
- Fresh, never frozen, incubated with antibody day of collection
- Live or Fixed
- Live at the time of antibody incubation
- Fixed after antibody incubation?
- No, no fix after antibody incubation
Contact the contributor via MetrdyWe pass your message on; contributors' addresses are not published.
Figure
A titer holds for the conditions it was run under: cells, instrument and staining conditions all change it. Metrdy checks each submission for completeness before publishing it, but does not validate the results.
Files
0.01.fcs, 0.02.fcs, 0.04.fcs, 0.078.fcs, 0.156.fcs, 0.3125.fcs, 0.625.fcs, 1.25.fcs, 2.5.fcs
Download ZIP (88.5 MB)DOI
https://doi.org/10.64157/GSYU1091Citation
Marvin, J. (2026). CD8 RV828 Titration Dataset (TiterPubs GSYU1091) [Data set]. Metrdy. https://doi.org/10.64157/GSYU1091
Used this titer? Tell other labs how it went. Download the report form, then upload it on the Contribute tab or email it to us. We review every report and add it here, signed with your name.